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Selection of HPLC methods in pharmaceutical analysis I. Optimization for selectivity in reversed phase chromatography
Summary
Type:
HPLC
Application #:
109370
Catalog#:
720023.46 - MN HPLC Column, Nucleosil C18, 100A, 10um, 4.6x250mm
Detection:
UV, 240nm
Conditions:
eluent: acetonitrile - tetrahydrofuran - water (12.9:22.4: 64.7)
flow rate 1ml/min
Authors:
Gazdag, M. et al.
Source:
J. Chromatogr. 454 (1988) 83–94
Details
Legend: Fig. 8. Optimized chromatogram of steroids, N = norethindrone, E = ethynylestradiol, NG = norgestrel, EO = estrone, NAC = norethindrone acetate, M = mestranol.
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